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firefly luciferase dna reporter construct  (Thermo Fisher)


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    Thermo Fisher firefly luciferase dna reporter construct
    Firefly Luciferase Dna Reporter Construct, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/firefly+luciferase+reporter+dna/DNA/pmc06528791-120-4-27
    Average 99 stars, based on 1 article reviews
    firefly luciferase dna reporter construct - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: Tetradecanol reduces EL-4 T cell growth by the down regulation of NF-κB mediated IL-2 secretion.
    Article Snippet: Tetradecanol is a straight-chain saturated fatty alcohol purified from Dendropanax morbifera leaves.. We found that tetradecanol (30 μM) reduced specifically the growth of T cells such as EL-4 T cell and isolated murine CD4 T cells.. In this study, we investigated the effects of tetradecanol on the regulation of interlukin-2 (IL-2), a potent T cell growth factor.

    Article Title: Peroxisome proliferator-activated receptor gamma (PPARgamma) suppresses colonic epithelial cell turnover and colon carcinogenesis through inhibition of the beta-catenin/T cell factor (TCF) pathway.
    Article Snippet: .. Transfections were performed in accordance with the manufacturer’s procedure using 3 μl of Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA), 2 μg of Firefly Luciferase Reporter DNA (TOPflash or control FOP flash; Upstate Biotechnology, Inc., New York, NY, USA), and 0.5 μg of phRL-TK-Renilla Luciferase (Promega, Madison, WI, USA) as a control for transfection efficiency in 200 μl of Opti-mem (Invitrogen). .. At 24 h after the transfection, the medium was removed and the cells were treated with medium containing or not containing 10 μM of PGJ2 for 8 h. The medium was removed and the Firefly and Renilla luciferase activities were sequentially measured using the Dual-Glo buffer system (Promega).

    Luciferase:

    Article Title: Tetradecanol reduces EL-4 T cell growth by the down regulation of NF-κB mediated IL-2 secretion.
    Article Snippet: Tetradecanol is a straight-chain saturated fatty alcohol purified from Dendropanax morbifera leaves.. We found that tetradecanol (30 μM) reduced specifically the growth of T cells such as EL-4 T cell and isolated murine CD4 T cells.. In this study, we investigated the effects of tetradecanol on the regulation of interlukin-2 (IL-2), a potent T cell growth factor.

    Article Title: Peroxisome proliferator-activated receptor gamma (PPARgamma) suppresses colonic epithelial cell turnover and colon carcinogenesis through inhibition of the beta-catenin/T cell factor (TCF) pathway.
    Article Snippet: .. Transfections were performed in accordance with the manufacturer’s procedure using 3 μl of Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA), 2 μg of Firefly Luciferase Reporter DNA (TOPflash or control FOP flash; Upstate Biotechnology, Inc., New York, NY, USA), and 0.5 μg of phRL-TK-Renilla Luciferase (Promega, Madison, WI, USA) as a control for transfection efficiency in 200 μl of Opti-mem (Invitrogen). .. At 24 h after the transfection, the medium was removed and the cells were treated with medium containing or not containing 10 μM of PGJ2 for 8 h. The medium was removed and the Firefly and Renilla luciferase activities were sequentially measured using the Dual-Glo buffer system (Promega).

    Control:

    Article Title: Peroxisome proliferator-activated receptor gamma (PPARgamma) suppresses colonic epithelial cell turnover and colon carcinogenesis through inhibition of the beta-catenin/T cell factor (TCF) pathway.
    Article Snippet: .. Transfections were performed in accordance with the manufacturer’s procedure using 3 μl of Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA), 2 μg of Firefly Luciferase Reporter DNA (TOPflash or control FOP flash; Upstate Biotechnology, Inc., New York, NY, USA), and 0.5 μg of phRL-TK-Renilla Luciferase (Promega, Madison, WI, USA) as a control for transfection efficiency in 200 μl of Opti-mem (Invitrogen). .. At 24 h after the transfection, the medium was removed and the cells were treated with medium containing or not containing 10 μM of PGJ2 for 8 h. The medium was removed and the Firefly and Renilla luciferase activities were sequentially measured using the Dual-Glo buffer system (Promega).



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    firefly luciferase dna reporter construct  (Thermo Fisher)
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    A comparison of the effects of stabilizing and destabilizing osmolytes versus that of heat shock in alleviating the diffuse mHtt dose-dependent repression of CRE-firefly <t>luciferase</t> reporter gene. The 14A2.6 line of PC12 cells were transfected with the CRE-firefly luciferase reporter <t>DNA</t> along with the internal control CMV-driven humanized Renilla luciferase reporter DNA as described . After transfection, cells were plated at t = 0 into a 96 well plate and ponasterone (0–5 µM) was added to designated wells to final concentrations shown. Osmolytes (120 mM) were added at t = 24 h and incubation continued at 37 °C. Heat shock of cells was for 2 h at 42 °C at t = 24 h followed by recovery at 37 °C. The CRE-reporter gene was induced by the addition of 1 mM dibutyryl cAMP at t = 48 h and incubation for an additional 6 h at 37 °C. Reporter gene activity was assayed using the Dual-Glo reporter gene assay kit from Promega Inc. Results on the firefly luciferase reporter gene activity was normalized against that of the Renilla luciferase relative to the ratio of 1 for the un-induced control (basal; without PA or dibutyryl cAMP induction). Result is the average from three separate experiment each with 8 independent determinations. Probability of difference P > 0.05 is defined as not significant, between 0.01 and 0.05 is significant (*), < 0.01 is very significant (**), and < 0.001 is extremely significant (***).
    Firefly Luciferase Dna Reporter Construct, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A comparison of the effects of stabilizing and destabilizing osmolytes versus that of heat shock in alleviating the diffuse mHtt dose-dependent repression of CRE-firefly luciferase reporter gene. The 14A2.6 line of PC12 cells were transfected with the CRE-firefly luciferase reporter DNA along with the internal control CMV-driven humanized Renilla luciferase reporter DNA as described . After transfection, cells were plated at t = 0 into a 96 well plate and ponasterone (0–5 µM) was added to designated wells to final concentrations shown. Osmolytes (120 mM) were added at t = 24 h and incubation continued at 37 °C. Heat shock of cells was for 2 h at 42 °C at t = 24 h followed by recovery at 37 °C. The CRE-reporter gene was induced by the addition of 1 mM dibutyryl cAMP at t = 48 h and incubation for an additional 6 h at 37 °C. Reporter gene activity was assayed using the Dual-Glo reporter gene assay kit from Promega Inc. Results on the firefly luciferase reporter gene activity was normalized against that of the Renilla luciferase relative to the ratio of 1 for the un-induced control (basal; without PA or dibutyryl cAMP induction). Result is the average from three separate experiment each with 8 independent determinations. Probability of difference P > 0.05 is defined as not significant, between 0.01 and 0.05 is significant (*), < 0.01 is very significant (**), and < 0.001 is extremely significant (***).

    Journal: Scientific Reports

    Article Title: Osmolytes dynamically regulate mutant Huntingtin aggregation and CREB function in Huntington’s disease cell models

    doi: 10.1038/s41598-020-72613-3

    Figure Lengend Snippet: A comparison of the effects of stabilizing and destabilizing osmolytes versus that of heat shock in alleviating the diffuse mHtt dose-dependent repression of CRE-firefly luciferase reporter gene. The 14A2.6 line of PC12 cells were transfected with the CRE-firefly luciferase reporter DNA along with the internal control CMV-driven humanized Renilla luciferase reporter DNA as described . After transfection, cells were plated at t = 0 into a 96 well plate and ponasterone (0–5 µM) was added to designated wells to final concentrations shown. Osmolytes (120 mM) were added at t = 24 h and incubation continued at 37 °C. Heat shock of cells was for 2 h at 42 °C at t = 24 h followed by recovery at 37 °C. The CRE-reporter gene was induced by the addition of 1 mM dibutyryl cAMP at t = 48 h and incubation for an additional 6 h at 37 °C. Reporter gene activity was assayed using the Dual-Glo reporter gene assay kit from Promega Inc. Results on the firefly luciferase reporter gene activity was normalized against that of the Renilla luciferase relative to the ratio of 1 for the un-induced control (basal; without PA or dibutyryl cAMP induction). Result is the average from three separate experiment each with 8 independent determinations. Probability of difference P > 0.05 is defined as not significant, between 0.01 and 0.05 is significant (*), < 0.01 is very significant (**), and < 0.001 is extremely significant (***).

    Article Snippet: The pCRE-firefly luciferase reporter DNA (E8471), humanized Renilla luciferase DNA (phRLSV40), and the Dual-Glo luciferase assay reagent (E2920) were from Promega Inc. Lipofectamine 2000 reagent used for DNA transfection was from Invitrogen Co.

    Techniques: Comparison, Luciferase, Transfection, Control, Incubation, Activity Assay, Reporter Gene Assay